Studies on biochemical effects of aqueous extract of Carica papaya leaf on alloxan-induced diabetic albino rats were undertaken. One hundred albino rats of Wistar strain were obtained and divided into five groups of twenty rats each and properly treated with the aqueous extract after four out of the five groups were induced with diabetes. The diabetic induced groups were diabetic control, test groups I (treated with 200 mg/kg) and II (treated with 50 mg/kg), and reference (treated with the standard glibenclamide). The diabetic free group was designated as normal control. Results obtained revealed that oral administration of aqueous extract of C. papaya leaf on alloxan-induced diabetic albino rats significantly reversed the damage associated with alloxan-induced diabetes revealing its hypoglycemic, liver and renal function integrity effects. The presence of phytochemicals and phytonutrients found in C. papaya could explain the observed pharmacological property of the studied extract. This study has revealed the biochemical effects of aqueous extract of C. papaya leaf on alloxan-induced diabetic albino rats.