Mango (Mangifera indica L.) is, as a king of fruit, dominating fruit in consumer point of due to its delicious taste and flavour in India. Being a largest producing country in the world, it has more than thousand varieties in biodiversity. Though, the production the production is very low as compare to other country. Hence, the study has been framed to evaluate thirteen varieties and seven hybrids of mango at ripe stage for their morphological, physical and biochemical characters were made under South Gujarat agro-climatic conditions at Navsari Agricultural University, Navsari. Improvement in mango through hybridization is a very tedious and long task as it has more than seven years of juvenile phase. For the goal oriented improvement work, systematic morphological characterization of genetic material is the basic and necessary work which is useful for the selection of parental materials in improvement program as well as identification of different varieties. The experimental results revealed that the mango varieties/hybrids expressed the morphological variations from genotype to genotype. Among these twenty genotypes, seven characters for fruit viz., fruit shape (obovoid, round, ovate, elliptical, oblong); color of ripen fruit (yellow, green with red blush, greenish yellow, bright orange, saffron yellow, apricot yellow, cadmium yellow, golden yellow); fiber content (fiberless, medium, low, high); pulp texture (intermediate, soft, firm); beak type (perceptible, pointed, mammiform); sinus type (shallow, prominent, absent) and fruit apex (obtuse, round, acute) were examined and documented. The cultivar Rajapuri had the maximum fruit weight (974.33 g), length (17.63 cm), thickness (12.73 cm), peel weight (87.77 g) while stone weight (57.57 g) was highest in cv Baneshan. The maximum content of pulp percentage was noted in cv Totapuri (89.61%). Among the chemical parameters, the maximum total soluble solids (TSS) was recorded in cv. Kesar (21.96 ºBrix). The maximum total sugars (19.34%), reducing sugars (6.08%), non-reducing sugars (12.88%) and minimum titrable acidity (0.17%) were recorded in cv Alphonso. The maximum ascorbic acid content was observed in cv Langra (55.73 mg/100g). Based on the overall performance of the mango cultivars Rajapuri, Baneshan and Totapuri were found better in physical properties whereas, Kesar, Alphonso and Langra exhibited their superiority in chemical