Incidence of subclincial mastitis (SCM) among crossbred cattle from organized dairy farms was studied. The quarter-wise and animal-wise incidence of SCM was 15.62 and 43.33 per cent, respectively. Maximum number of animals (74.36%) were having one quarter infection. Right-hind quarters were having higher incidence (38.18%) compared with other quarters. The incidence of SCM was lowest during first lactation which increased with subsequent lactations and was highest in sixth and above lactations. Bacteriological isolations revealed that Staphylococcus aureus was the major pathogen (56.89%) followed by Micrococcus spp. (15.51%) Bacillus spp. (12.06%), Staphylococcus epidermidis (8.62%), Klebsiella spp (3.44%), E. coli (1.72%) and Corynebacterium spp. (1.72%). Among the indirect tests, SLS was found to be highly (19.03%) effective in detection of subclinical mastitis followed by WST, BTB Card test and CMT (15.90, 15.62 and 14.77%, respectively). The percentage of agreement of CMT, SLS, BTB Card test and WST with bacteriological examination were found to be 57.89, 62.07, 64.46 and 68.65 per cent, respectively.