The aim of the present study was to assess the compliance of young dental practitioners in Iasi, Romania, regarding protective gloves. Material and methods Cross-sectional, questionnaire-based study included 70 students attending the years 4, 5 and 6 of the Faculty of Dental Medicine within the “Grigore T. Popa” University of Medicine and Pharmacy in Iasi. Self-administered questionnaire contained 10 questions with closed answer, single or multiple. Results 83.33% of the respondents used protective gloves for all the patients and procedures, 55% of them washed their hands before and after gloves application and 60% presented intolerance reactions to latex gloves. Conclusion Compliance of the investigated dental students with wearing protective gloves can be considered good, but additional training programs on infection control measures for young practitioners would be useful in order to protect patients and also to benefit from safe working conditions.