[HTML][HTML] Substituted diaryl diselenides: Cytotoxic and apoptotic effect in human colon adenocarcinoma cells
Life Sciences, 2012•Elsevier
AIMS: To investigate the effects and study the underlying cell death mechanisms of diaryl
diselenides, including: diphenyl diselenide (C6H5Se) 2; 4-chlorodiphenyl diselenide (4-
ClC6H4Se) 2; 3-(trifluoromethyl)-diphenyl diselenide (3-CF3C6H4Se) 2 and 4-
methoxydiphenyl diselenide (4-MeOC6H4Se) 2, on the human colon adenocarcinoma cell
line HT-29. MAIN METHODS: The viability of HT-29 cells after exposure to the diaryl
diselenides and its substituted structures was based on the MTT assay. To verify if cell death …
diselenides, including: diphenyl diselenide (C6H5Se) 2; 4-chlorodiphenyl diselenide (4-
ClC6H4Se) 2; 3-(trifluoromethyl)-diphenyl diselenide (3-CF3C6H4Se) 2 and 4-
methoxydiphenyl diselenide (4-MeOC6H4Se) 2, on the human colon adenocarcinoma cell
line HT-29. MAIN METHODS: The viability of HT-29 cells after exposure to the diaryl
diselenides and its substituted structures was based on the MTT assay. To verify if cell death …
AIMS
To investigate the effects and study the underlying cell death mechanisms of diaryl diselenides, including: diphenyl diselenide (C6H5Se)2; 4-chlorodiphenyl diselenide (4-ClC6H4Se)2; 3-(trifluoromethyl)-diphenyl diselenide (3-CF3C6H4Se)2 and 4-methoxydiphenyl diselenide (4-MeOC6H4Se)2, on the human colon adenocarcinoma cell line HT-29.
MAIN METHODS
The viability of HT-29 cells after exposure to the diaryl diselenides and its substituted structures was based on the MTT assay. To verify if cell death was mediated throughout apoptosis mechanisms, flow cytometry and real-time PCR (qPCR) analyses were conducted.
KEY FINDINGS
The MTT assay and flow cytometry analyses showed that (3-CF3C6H4Se)2 and (4-MeOC6H4Se)2 induced cytotoxicity through apoptosis mechanisms in HT-29 cells. qPCR revealed there was an up-regulation of pro-apoptotic (Bax, casapase-9, caspase-8, apoptosis-inducing factor (AIF) and Endonuclease G (EndoG)) and cell-cycle arrest genes (p53 and p21) and down-regulation of anti-apoptotic (Bcl-2 and survivin) and Myc genes.
SIGNIFICANCE
These results demonstrate that (3-CF3C6H4Se)₂ and (4-MeOC6H4Se)2 have the potential to induce apoptosis in HT-29 cells through the activation of caspase-dependent and independent pathways and through cell-cycle arrest.
Elsevier