Background: Hepatitis B virus infection is a major global health problem of public health importance. The World Health Organization (WHO) African Region has reported an approximately 100 million people with chronic hepatitis B virus (HBV) infection and Nigeria has been classified as endemic.

Objectives: The objectives of this study were to determine (i) the prevalence of hepatitis B infection (ii) the immune status to hepatitis B among patients (iii) the susceptibility rate. Methods: A total of four hundred and ten (410) sera were collected at random from patients attending various hospitals in Borno state. A self-structured questionnaire was used to obtained demographic data which included age and sex, social status (eg trader, farmer, civil servant or student), pregnancy status, blood donor type. Sera samples were obtained for qualitative detection of HBsAg using rapid chromatographic immunoassays with test kits from Qingdao Hightop Biotech Co., China having sensitivity, specificity and accuracy of 99%, 97% and 98.5% respectively. Results: This is a first-phase result of a surveillance of hepatitis B biomarkers in Borno state Nigeria. The proportion of male (248: 60.5%) was higher than female (162: 39.5%). Out of the 410