Synthesis and evaluation of a series of 6-chloro-4-methylumbelliferyl glycosides as fluorogenic reagents for screening metagenomic libraries for glycosidase activity
Screening of large enzyme libraries such as those derived from metagenomic sources
requires sensitive substrates. Fluorogenic glycosides typically offer the best sensitivity but
typically must be used in a stopped format to generate good signal. Use of fluorescent
phenols of pKa< 7, such as halogenated coumarins, allows direct screening at neutral pH.
The synthesis and characterisation of a set of nine different glycosides of 6-chloro-4-
methylumbelliferone are described. The use of these substrates in a pooled format for …
requires sensitive substrates. Fluorogenic glycosides typically offer the best sensitivity but
typically must be used in a stopped format to generate good signal. Use of fluorescent
phenols of pKa< 7, such as halogenated coumarins, allows direct screening at neutral pH.
The synthesis and characterisation of a set of nine different glycosides of 6-chloro-4-
methylumbelliferone are described. The use of these substrates in a pooled format for …
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