The present work reports the synthesis of bioactive calcium phosphate from cockle shell via the combination of calcination and hydrothermal process. The raw cockle shells were pre-treated with 30 % of hydrogen peroxide for 4 days to eliminate the impurities. Afterward, the dried cockle shells were crushed and calcined at various temperatures ranging from 300 to 1100 °C. Subsequently, the calcined powders underwent hydrothermal process in di-ammonium hydrogen phosphate and distilled water at pH of 10.5 for 30 minutes. Lastly, the hydrothermal treated powders were dried in oven at 50 °C for 3 days. The results showed that the mixture of aragonite, calcite, hydroxyapatite, and calcium hydroxide was successfully synthesized at a calcination temperature of 900 °C and 1100 °C. In addition, the nanorods in the length of 80-300 nm were formed. The findings of this work indicate that the cockle shell could be transformed into valuable bioactive materials for biomedical applications.