Synthetic biology expands the industrial potential of Yarrowia lipolytica

KA Markham, HS Alper - Trends in biotechnology, 2018 - cell.com
Trends in biotechnology, 2018cell.com
The oleaginous yeast Yarrowia lipolytica is quickly emerging as the most popular non-
conventional (ie, non-model organism) yeast in the bioproduction field. With a high
propensity for flux through tricarboxylic acid (TCA) cycle intermediates and biological
precursors such as acetyl-CoA and malonyl-CoA, this host is especially well suited to meet
our industrial chemical production needs. Recent progress in synthetic biology tool
development has greatly enhanced our ability to rewire this organism, with advances in …
The oleaginous yeast Yarrowia lipolytica is quickly emerging as the most popular non-conventional (i.e., non-model organism) yeast in the bioproduction field. With a high propensity for flux through tricarboxylic acid (TCA) cycle intermediates and biological precursors such as acetyl-CoA and malonyl-CoA, this host is especially well suited to meet our industrial chemical production needs. Recent progress in synthetic biology tool development has greatly enhanced our ability to rewire this organism, with advances in genetic component design, CRISPR technologies, and modular cloning strategies. In this review we investigate recent developments in metabolic engineering and describe how the new tools being developed help to realize the full industrial potential of this host. Finally, we conclude with our vision of the developments that will be necessary to enhance future engineering efforts.
cell.com
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