Systematic application of multiplex PCR enhances the detection of bacteria, parasites, and viruses in stool samples
GN McAuliffe, TP Anderson, M Stevens, J Adams… - Journal of Infection, 2013 - Elsevier
OBJECTIVES: To determine whether systematic testing of faecal samples with a broad
range multiplex PCR increases the diagnostic yield in patients with diarrhoea compared with
conventional methods and a clinician initiated testing strategy. METHODS: 1758 faecal
samples from 1516 patients with diarrhoea submitted to two diagnostic laboratories were
tested for viral, bacterial, and parasitic pathogens by Fast-Track Diagnostics multiplex real-
time PCR kits and conventional diagnostic tests. RESULTS: Multiplex PCR detected …
range multiplex PCR increases the diagnostic yield in patients with diarrhoea compared with
conventional methods and a clinician initiated testing strategy. METHODS: 1758 faecal
samples from 1516 patients with diarrhoea submitted to two diagnostic laboratories were
tested for viral, bacterial, and parasitic pathogens by Fast-Track Diagnostics multiplex real-
time PCR kits and conventional diagnostic tests. RESULTS: Multiplex PCR detected …
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