The bioprocess methodology for the enhanced production of phytochemicals using plant cultures predominantly depends on the elicitors and precursors. The drug L-DOPA (anti-Parkinson's drug) (L-3, 4-dihydroxyphenylalanine) has been chiefly isolated from the popular medicinal plant species Mucuna. The present study has been carried out to enhance the production of L-DOPA and other secondary metabolites in the presence of the optimized concentration of growth promoters, elicitors and precursor's in vitro callus culture of Mucuna imbricata. Results revealed that there is a positive influence of Methyl Jasmonate (MJC) and Salicylic Acid (SAC) on the accumulation of L-DOPA and other secondary metabolites (Nutritional and anti-nutritional factors), which enhances their production by more than two folds. The highest accumulation of L-DOPA was noticed in the elicitation of 1 mM MJC (4.3 ± 0.4 %) followed by 1 mM SAC (3.4 ± 0.08 %) on 45^th and 30^th days respectively. Results also illustrated a significant increase of callus biomass (dry and moist weight) as well as total phenolics, flavonoids and antioxidants values in the elicited callus from day 30^th to day 45^th of explant inoculation which indicates a synergistic interaction of auxin, cytokinins with elicitors and precursors respectively. Additionally, results revealed that improved accumulation of carbohydrates was positively correlated with an increase in L-DOPA content in the elicited callus. Hence the protocol formulated delivers an effective alternative for mass production of L-DOPA from callus culture with improved metabolite content.