The foregoing areal expansion of Dhaka city with its dense population has been triggered the processes of land transformation as well as the growth of urbanization that are responsible for the physical and environmental instability of that area. In this research attempt has been taken to detect the loss of vegetation cover for the Dhaka district using multitemporal, multidate and multisensor aerospace data and to analyse the assessment of the impact of urbanization and biodiversity. Landsat imageries of the year January 1989, February 2002 and January 2010 have been used to find out the difference of vegetation coverage of Dhaka metropolitan and its surroundings over 20 years. About 20 percent vegetation cover that was present in 1989 has gradually decreased to 15.5 and 7.3 percent in year 2002 and 2010, respectively. Abrupt declination of vegetation coverage has been identified in Dhaka Metropolitan and Savar thana with a rate of 3.5 and 2.72 sqkm per year since last two decades, whereas gradual loss has been found in Nawabganj thana with an average of 1.62 sqkm per year. A considerably better situation has been observed in Keraniganj, Dhamrai and Dohar thana where the increasing rate was 0.9 sqkm during 1989–2002 period and declined at a rate of 2.28 sqkm during 2002–2010. The research has shown the pattern and nature of the interrelationship between urban sprawl and urban vegetation loss. Temporal urban map and database provides the baseline information for the planner and practitioners to monitor and predict the patterns and future trends of urbanization. Temporal mapping is driven by remote sensing and Geographic Information System (GIS) to capture and analyse information from both historical and modern records. The map and the database have been focused the intense changes to the landscape that have incrementally developed over time.