The biocide efficiency of bioactive 45S5 Bioglass® derived glass‐ceramic substrates against common Gram positive and Gram negative bacteria, and also against yeast, is investigated in this study. The results confirm the high bactericidal capacity of this material. Inorganic coatings containing silver nanoparticles on glass‐ceramic substrates are developed in order to ascertain whether the presence of silver enhances the biocide activity. It is found that a coating made of soda‐glass containing silver nanoparticles does not improve the antifungal properties of the 45S5 Bioglass® derived glass‐ceramic substrates.