With the rapid development of wireless communication technology and the proliferation of mobile devices, mobile applications are more and more widely used. Depending on the characteristics that the existing mobile applications need long development cycles depending on a variety of mobile devices, this paper puts forward an HTML5-based mobile middleware on the basis of the traditional middleware and mobile middleware. The mobile middleware uses the B/C/S hybrid architecture and shields the differences between the hardware and software of mobile devices, which enables developers to develop applications that are suitable for mobile terminal platforms according to MVC pattern. Compared with other middlewares, HTML5-based mobile middleware has the advantages of short develop cycle, low cost and low difficulty as well as good expansibility.