The pathophysiology of contrast-induced AKI (CIAKI) is incompletely understood due to the
lack of an appropriate in vivo model that demonstrates reduced kidney function before
administration of radiocontrast media (RCM). Here, we examine the effects of CIAKI in vitro
and introduce a murine ischemia/reperfusion injury (IRI)–based approach that allows
induction of CIAKI by a single intravenous application of standard RCM after injury for in vivo
studies. Whereas murine renal tubular cells and freshly isolated renal tubules rapidly …