The aim of this research was to determine the effect of the level of phosphorus fertilizer on the growth and dry matter production Stylosanthes guianensis. This research aimed to know the effect of phosphorus fertilizer application on growth and dry matter production of stylo (Stylosanthes guianensis). This research is based on a completely randomized design with four treatments and four replicates to obtain 16 experimental units. The treatments of this research are P0 (control), P1 (0.75 g TSP/polybag), P2 (1.5 g TSP/polybag), P3 (2.25 g TSP/polybag). The parameters were plant height, number of leaf branches, number of branches plant, dry matter production, and the number of nodules. The results showed that the treatments have not significantly affected (P> 0.05) plant height, number of stems, number of branches, and dry matter production, but it had a highly significant effect (P< 0.01) on the number of nodules. However, P3 treatment was better to use than the other treatments.