Biodiesel is an alternative fuel that recently widely used to substitute fossil fuels. Biodiesel production from vegetable oil produces glycerol as by-product which is a very promising low-cost feedstock for producing a wide variety of special and fine chemicals. One of the potential glycerol derivatives is triacetin, a promising alternative chemical to be fuel additive. In this work, triacetin was synthesized using reactive distillation with recycle stream from bottom product to increase the conversion of monoacetin became diacetin and diacetin became triacetin. The effects of using catalyst and recycle stream are evaluated. The experiments showed recycle stream made contact between reactant were better. So the selectivity and the yield increased. The optimum recycle rate is 65.5 mL/men and the results are 98.38% of glycerol conversion and 9.00% of triacetin yield.