This study investigated the effects of a mentally fatiguing test on physical tasks among elite cricketers. In a cross-over design, 10 elite male cricket players from a professional club performed a cricket run-two test, a Batak Lite reaction time test and a Yo-Yo-Intermittent Recovery Level 1 (Yo-Yo-IR1) test, providing a rating of perceived exertion (RPE) after completing a 30-min Stroop test (mental fatigue condition) or 30-min control condition. Perceived fatigue was assessed before and after the two conditions and motivation was measured before testing. There were post-treatment differences in the perception of mental fatigue (P < 0.001; d = −7.82, 95% CIs = −9.05–6.66; most likely). Cricket run-two (P = 0.002; d = −0.51, 95% CIs = −0.72–0.30; very likely), Yo-Yo-IR1 distance (P = 0.023; d = 0.39, 95% CIs = 0.14–0.64; likely) and RPE (P = 0.001; d = −1.82, 95% CIs = −2.49–1.14; most likely) were negatively affected by mental fatigue. The Batak Lite test was not affected (P = 0.137), yet a moderate (d = 0.41, 95% CIs = −0.05–0.87) change was likely. Mental fatigue, induced by an app-based Stroop test, negatively affected cricket-relevant performance.