The epithelial sodium channel (ENaC) traffics to apical membrane in lipid rafts in mouse cortical collecting duct cells
We previously showed that ENaC is present in lipid rafts in A6 cells, a Xenopus kidney cell
line. We now demonstrate that ENaC can be detected in lipid rafts in mouse cortical
collecting duct (MPK CCD 14) cells by detergent insolubility, buoyancy on density gradients
using two distinct approaches, and colocalization with caveolin 1. Less than 30% of ENaC
subunits were found in raft fractions. The channel subunits also colocalized on sucrose
gradients with known vesicle targeting and fusion proteins syntaxin 1A, Vamp 2, and …
line. We now demonstrate that ENaC can be detected in lipid rafts in mouse cortical
collecting duct (MPK CCD 14) cells by detergent insolubility, buoyancy on density gradients
using two distinct approaches, and colocalization with caveolin 1. Less than 30% of ENaC
subunits were found in raft fractions. The channel subunits also colocalized on sucrose
gradients with known vesicle targeting and fusion proteins syntaxin 1A, Vamp 2, and …
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