This study aims to identify the impact of organizational ambidexterity on strategic orientations and the mediating role of organizational innovation in private hospitals in Jordan. A questionnaire used as a tool to collect data through an investigation of the opinions of a sample of 274 managers. The study arrived at the following results: Organizational ambidexterity is of moderate importance, higher importance in strategic orientations, and moderate importance in organizational innovation in private hospitals in Jordan. Second, there is a statistically significant impact at (P≤ 0.05) for organizational ambidexterity with its dimensions on the strategic orientations with their combined dimensions in private hospitals in Jordan. Finally, there is a statistically significant impact at (P≤ 0.05) for organizational ambidexterity with its dimensions on the strategic orientations with their combined dimensions in the presence of organizational innovation in private hospitals in Jordan. Accordingly, the study recommended the following: The upper administration in hospitals need to work on promoting the concept of organizational ambidexterity. The focus is on the process of creating balance between exploring opportunities and exploiting them. In addition, hospital management should take care of the staff by achieving innovation and encouraging the creation and prioritization of an innovative culture. Management needs also to carry out research on creative individuals in order to unify them, monitor and keep pace with future changes, and improve the sustainability of hospital work.