The existence of mass transportation is the crux of development. Improving the citizen satisfaction in this sector must be done by all of related parties. The main purposes of this research were to determine and to analyze the impact of work culture and service quality on citizen satisfaction in mass transportation in South Pesisir Region. This research used the quantitative method. The research population was citizen of South Pesisir Region. Sampling method used for this research was Slovin formula and analysis techniques of proportionate stratified random sampling. The data were collected by using questionnaire with Likert scale and were analyzed by using multiple regression and documentation.