[HTML][HTML] The production and secretion of complement component C1q by human mast cells

RA van Schaarenburg, J Suurmond, KLL Habets… - Molecular …, 2016 - Elsevier
RA van Schaarenburg, J Suurmond, KLL Habets, MC Brouwer, D Wouters, FAS Kurreeman…
Molecular immunology, 2016Elsevier
C1q is the initiation molecule of the classical pathway of the complement system and is
produced by macrophages and immature dendritic cells. As mast cells share the same
myeloid progenitor cells, we have studied whether also mast cells can produce and secrete
C1q. Mast cells were generated in vitro from CD34+ progenitor cells from buffy coats or cord
blood. Fully differentiated mast cells were shown by both RNA sequencing and qPCR to
express C1QA, C1QB and C1QC. C1q produced by mast cells has a similar molecular make …
Abstract
C1q is the initiation molecule of the classical pathway of the complement system and is produced by macrophages and immature dendritic cells. As mast cells share the same myeloid progenitor cells, we have studied whether also mast cells can produce and secrete C1q.
Mast cells were generated in vitro from CD34+ progenitor cells from buffy coats or cord blood. Fully differentiated mast cells were shown by both RNA sequencing and qPCR to express C1QA, C1QB and C1QC. C1q produced by mast cells has a similar molecular make-up as serum C1q. Reconstituting C1q depleted serum with mast cell supernatant in haemolytic assays, indicated that C1q secreted by mast cells is functionally active. The level of C1q in supernatants produced under basal conditions was considerably enhanced upon stimulation with LPS, dexamethasone in combination with IFN- γ or via FcεRI triggering. Mast cells in human tissues stained positive for C1q in both healthy and in inflamed tissue. Moreover, mast cells in healthy and diseased skin appear to be the predominant C1q positive cells.
Together, our data reveal that mast cells are able to produce and secrete functional active C1q and indicate mast cells as a local source of C1q in human tissue.
Elsevier
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