This study aimed at developing an attitude scale for biology course. Study samples were composed of 169 students to have attended Science High School in Kırklareli Province, Turkey, in 2008-2009 education year. In the scope of the present study, the scale developed by Demirci (2003) for the science course was adopted to biology course. The scale (which was a one-dimension in its original format) was subjected to factor analysis, which showed that adopted scale was also a one-factor scale with factor loads varying in .40-.75 range. Cronbach alpha reliability coefficient of this 32-item attitude scale for biology course was calculated to be .96.