Thetms2 gene as a negative selection marker in rice
Plant Molecular Biology Reporter, 2000•Springer
A conditional negative selection marker is essential for high throughput insertional
mutagenesis with any two-element transposon tagging system. The tms2 gene encodes
indoleacetic acid hydrolase (IAAH) which converts naphthaleneacetamide (NAM) to the
potent auxin naphthaleneacetic acid, a phytotoxic derivative. This gene, under the control of
the manopine synthase gene 2 promoter from Agrobacterium tumefaciens and exogenously
applied NAM, have been used effectively as a negative selector in Ac/Ds insertional …
mutagenesis with any two-element transposon tagging system. The tms2 gene encodes
indoleacetic acid hydrolase (IAAH) which converts naphthaleneacetamide (NAM) to the
potent auxin naphthaleneacetic acid, a phytotoxic derivative. This gene, under the control of
the manopine synthase gene 2 promoter from Agrobacterium tumefaciens and exogenously
applied NAM, have been used effectively as a negative selector in Ac/Ds insertional …
Abstract
A conditional negative selection marker is essential for high throughput insertional mutagenesis with any two-element transposon tagging system. Thetms2 gene encodes indoleacetic acid hydrolase (IAAH) which converts naphthaleneacetamide (NAM) to the potent auxin naphthaleneacetic acid, a phytotoxic derivative. This gene, under the control of the manopine synthase gene 2 promoter fromAgrobacterium tumefaciens and exogenously applied NAM, have been used effectively as a negative selector inAc/Ds insertional mutagenesis ofArabidopsis thaliana (Sundaresan et al., 1995). In this study we show thattms2 can also be used as a negative selector in rice. T1 transgenic seedlings expressing thistms2 gene under the control of themas2’ promoter showed significant reduction in shoot and root growth in the presence of 5–10 μM NAM under specified growth conditions compared to plants not containing this gene.
Springer
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