The use of a Spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male …
A Jagannath, P Bandyopadhyay, N Arumugam… - Molecular …, 2001 - Springer
Male-sterile lines were generated in oilseed mustard (Brassica juncea) with a cytotoxic gene
(barnase) in conjunction with either of two tapetum-specific promoters, TA29 and A9.
Several transformation vectors based on different promoter and marker gene combinations
were developed and tested for their efficacy in generating agronomically viable male-sterile
lines. Use of strong constitutive promoters (eg CaMV 35S or its double-enhancer variant) to
express the marker gene (bar) in barnase constructs generated male-sterile plants at an …
(barnase) in conjunction with either of two tapetum-specific promoters, TA29 and A9.
Several transformation vectors based on different promoter and marker gene combinations
were developed and tested for their efficacy in generating agronomically viable male-sterile
lines. Use of strong constitutive promoters (eg CaMV 35S or its double-enhancer variant) to
express the marker gene (bar) in barnase constructs generated male-sterile plants at an …
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