Thermo-separating polymer-based aqueous two-phase systems for the recovery of PEGylated lysozyme species
G Hernandez-Vargas, M González-González… - … of Chromatography B, 2019 - Elsevier
Fractionation of native, mono and di-PEGylated lysozyme was performed in 36 different
polymer-polymer aqueous two-phase systems using UCON as a phase-forming component.
After a discrete partition analysis, dextran 75 kDa-UCON, volume ratio 3, tie-line length 35%
w/w; ficoll 70 kDa-UCON, volume ratio 1, tie-line length 45% w/w and a PEG 8 kDa-UCON
volume ratio 3, tie-line length 65% w/w systems were selected for optimization via salt
addition and to observe the behavior of the lysozyme species in mixtures. The dextran …
polymer-polymer aqueous two-phase systems using UCON as a phase-forming component.
After a discrete partition analysis, dextran 75 kDa-UCON, volume ratio 3, tie-line length 35%
w/w; ficoll 70 kDa-UCON, volume ratio 1, tie-line length 45% w/w and a PEG 8 kDa-UCON
volume ratio 3, tie-line length 65% w/w systems were selected for optimization via salt
addition and to observe the behavior of the lysozyme species in mixtures. The dextran …
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