Although nanoparticles synthesized by chemical reactions show a wide range of toxicities in various vertebrates and invertebrates, they are produced in large quantities to take advantage of their antifungal activities. Due to the remarkable disadvantages of conventional chemical methods, the green synthesis of metallic nanoparticles has acquire great attention in recent years. This method is proposed as an alternative to classical chemical methods with its low cost, environmentally friendly and simple approaches. Our aim in this study is to determine the suitability of Tilia rubra DC. extract in the extracellular synthesis of silver nanoparticles and characterize the material obtained. T. rubra extract has been studied for the first time for the biosynthesis of silver nanoparticles, forming surface plasmon resonance at 427 nm in UV-Vis spectrophotometer. Also, transmission electron microscope (TEM) images showed that nanoparticles have spherical morphology. According to TEM images, nanoparticles were found to be between 5-15 nm in size. X-ray diffraction (XRD) analysis, on the other hand, determined that the particles were crystalline with a face centered cubic geometry. Fourier transform infrared (FTIR) analysis was performed to identify possible biomolecules responsible for bio-reduction of silver ions. The antifungal effect of the biosynthesis silver nanoparticles on Candida albicans, a pathogenic yeast, was examined by the agar diffusion method, and as a result, the synthesized silver nanoparticles showed a significant inhibitory effect on C. albicans. Our results support the potential for future use of biosynthesized silver nanoparticles for industrial and biomedical applications, thanks to the environmentally friendly synthesis procedure.