Aflatoxins are hepatotoxic metabolites and found as contaminants in various agricultural commodities such as maize, rice, sorghum, wheat, oats and various spices. In this study, Aflatoxin total (AFT) contamination of maize were evaluated by high performance liquid chromatography (HPLC). A total of 54 samples of maize were randomly collected from maize farm of different city of Qazvin, Iran. All maize samples were contaminated with AFT. the average concentrations of AFT: All corn samples studied in Qazvin province was 2.79±0.17 ng/g. In addition, the eight number (14.81%) of maize (21.42% of Boeen Zahra samples, 7.14% of Takestan samples and 28.57% of Qazvin samples), contamination rate were higher than of maximum tolerat level (MLT, 4 ng/g). Effective controlling of all food and foodstuffs which are vulnerable to AF contamination is necessary to prevent its effects.