Two-dimensional polyacrylamide gel electrophoresis of bovine seminal plasma proteins and their relation with semen freezability
MIM Jobim, ER Oberst, CG Salbego, DO Souza… - Theriogenology, 2004 - Elsevier
The objective of this study was to evaluate the low weight (10–30kDa) protein profile of
bovine seminal plasma using two-dimensional polyacrylamide gel electrophoresis (2D-
PAGE) and to determine if any of these proteins was associated with semen freezability.
Seminal plasma was collected from 16 bulls of high or low semen freezability. Twelve
protein spots were identified from the 2D gel (15%); six of these were present in all samples.
Of the 12 proteins found, three spots, present in all samples, 3 (15–16kDa), 5 (16–17kDa) …
bovine seminal plasma using two-dimensional polyacrylamide gel electrophoresis (2D-
PAGE) and to determine if any of these proteins was associated with semen freezability.
Seminal plasma was collected from 16 bulls of high or low semen freezability. Twelve
protein spots were identified from the 2D gel (15%); six of these were present in all samples.
Of the 12 proteins found, three spots, present in all samples, 3 (15–16kDa), 5 (16–17kDa) …
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