Unbiased strain-typing of arbovirus directly from mosquitoes using nanopore sequencing: a field-forward biosurveillance protocol
JA Russell, B Campos, J Stone, EM Blosser… - Scientific Reports, 2018 - nature.com
The future of infectious disease surveillance and outbreak response is trending towards
smaller hand-held solutions for point-of-need pathogen detection. Here, samples of Culex
cedecei mosquitoes collected in Southern Florida, USA were tested for Venezuelan Equine
Encephalitis Virus (VEEV), a previously-weaponized arthropod-borne RNA-virus capable of
causing acute and fatal encephalitis in animal and human hosts. A single 20-mosquito pool
tested positive for VEEV by quantitative reverse transcription polymerase chain reaction (RT …
smaller hand-held solutions for point-of-need pathogen detection. Here, samples of Culex
cedecei mosquitoes collected in Southern Florida, USA were tested for Venezuelan Equine
Encephalitis Virus (VEEV), a previously-weaponized arthropod-borne RNA-virus capable of
causing acute and fatal encephalitis in animal and human hosts. A single 20-mosquito pool
tested positive for VEEV by quantitative reverse transcription polymerase chain reaction (RT …
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