Use of DNA metabarcoding of bird pellets in understanding raptor diet on the Qinghai-Tibetan Plateau of China
Background Diet analysis is essential to understanding the functional role of large bird
species in food webs. Morphological analysis of regurgitated bird pellet contents is time
intensive and may underestimate biodiversity. DNA metabarcoding has the ability to
circumvent these issues, but has yet to be done. Methods We present a pilot study using
DNA metabarcoding of MT-RNR1 and MT-CO1 markers to determine the species of origin
and prey of 45 pellets collected in Qinghai and Gansu Provinces, China. Results We …
species in food webs. Morphological analysis of regurgitated bird pellet contents is time
intensive and may underestimate biodiversity. DNA metabarcoding has the ability to
circumvent these issues, but has yet to be done. Methods We present a pilot study using
DNA metabarcoding of MT-RNR1 and MT-CO1 markers to determine the species of origin
and prey of 45 pellets collected in Qinghai and Gansu Provinces, China. Results We …
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