This study evaluated the effectiveness of Moringa oleifera (M. oleifera) seed powder (MOSP) alone and in combination with household sand filter (MOSP+ F) in water purification. Treatment with MOSP+ F produced 99.97% reduction in E. coli compared with 98.16% reduction obtained from MOSP treatment alone. Water treatment with M. oleifera seed powder in combination with household sand filter was found to be more effective for water purification than treatment with Moringa seed powder alone. This method should be encouraged in communities without safe water supply.