In the present pot experiment, T. hamatum alone and in combination with rhizobial isolates was investigated their effects on growth of sunflower besides determining its effect on organic and inorganic constituents in leaves of same plant. The observations showed that T. hamatum itself and rhizobial isolates viz., JUR1, JUR2 and JUR4 significantly increased the lengths of both root & shoot and biomass as compared to control, same isolates in combination with T. hamatum were also found effective in promoting growth at 30th and 60th day of growth (p< 0.05). Total chlorophyll content was found significantly increased by T. hamatum, JUR1, JUR3, fertilizer and fungicide in their respective groups where as T. hamatum with JUR3, JUR4 and fertilizer increased same parameter at 30th day (p< 0.05). However, JUR1 alone and JUR4 with T. hamatum found to maintain the increase in chlorophyll content up to 60th day (p< 0.05). Similarly total carbohydrate and crude protein contents of sunflower leaves were increased by T. hamatum, JUR1 and JUR4 individually and T. hamatum with JUR3 and JUR4 at 30th day though the increase in these parameters was observed at 60th day of growth by JUR4 alone and JUR4 with T. hamatum (p< 0.05). T. hamatum, JUR1 and JUR4 individually and T. hamatum with JUR1, JUR3 and JUR4 significantly increased nitrogen content at both day intervals (p< 0.05). T. hamatum alone and with JUR1, JUR3 at 30th day while with JUR4 at 60th day of growth significantly increased phosphorus content (p< 0.05). The results concluded that T. hamtum alone and in combination with rhizobial isolates found effective not only increasing the growth of sunflower plant by improving its root-shoot length and biomass but also increasing the organic and inorganic content of same plant.