Implicit gender bias has costly and complex consequences for women in the workplace, with many women reporting gender microaggressions which result in them being overlooked or disrespected. We present an online desktop virtual environment that follows the story of a male or female self-avatar from the first-person perspective, who either experiences a positive or negative workplace scenario. The negative scenario included many examples from the taxonomy of gender microaggressions. Participants who experienced negative workplace experiences with a female self-avatar had significantly decreased levels of implicit gender bias compared to those who had a male self-avatar. There was evidence of empathy and perspective taking in the negative condition for the female self-avatar. Experiences of a positive workplace scenario showed no significant decreases in implicit gender bias regardless of self-avatar gender. We discuss the implications of these findings and make recommendations for virtual environment technologies and scenarios with respect to the reduction of implicit biases.