This paper describes a series of ambient vibration tests (AVTs) and modal analysis conducted on Huntington Bridge, located in Abbotsford, in the province of British Columbia, Canada. This multi-span bridge is composed of precast reinforced concrete (R/C) girders sitting on two R/C pairs at the middle and on abutments at the ends, which has been constructed in one of the most important transportation highways across east-west Canada. Modal response analysis was performed to determine the dynamic properties of the structure, including predominant natural frequencies and the corresponding mode shapes to calibrate the finite elements model of the bridge. The testing program consisted of several setups on different locations of the bridge including the deck and the abutments. A series of Tromino® velocity/acceleration wireless sensors was used to carry out the vibration measurements. The sensors were placed on predetermined locations according to the test plan. The collected records were time synchronized with a radio antenna and amplifier in each sensor. This allowed the synchronization of the recordings both within each measurement setup and between setups. The computer program ARTeMIS version 4, was used to perform the system identification of the structure. The software allows to develop a 3D model of the structure and test points; the resulting mode shapes are displayed using this geometry. Two techniques were used for modal identification: The Frequency Domain Decomposition (FDD), and the Stochastic Subspace Identification (SSI). These two modal identification techniques were used to cross-validate the results. The joint analysis of the signals measured in various strategic points of the structure made it possible to identify the modal configurations and the corresponding natural frequencies. A finite elements model was created using SAP2000 Software to simulate the dynamic behaviour of the bridge. The boundary conditions of the deck were modeled through rotational springs on the edges which were updated based on the modal responses of the bridge obtained from AVTs. As the results of this study, the natural frequencies and corresponding mode shapes of the bridge from AVTs were presented and compared with dynamic properties obtained from analytical model. The calibrated model was used to estimate the effect of earthquake damage on the load distribution in the bridge after an event. The redistributed loads were then computed and compared with the load capacity of the elements. This procedure can be used for rapid safety assessment of the bridge after earthquake to assure the safety of continuing the use of the bridge or to stop its operation.