[HTML][HTML] Using magnetic nanoparticles to probe protein damage in ferritin caused by freeze concentration
We demonstrate a method for monitoring the damage caused to a protein during freeze-
thawing in the presence of glycerol, a cryo-protectant. For this work we synthesized
magnetite nanoparticles doped with 2.5% cobalt inside the protein ferritin (CMF), dissolved
them in different concentration glycerol solutions and measured their magnetization after
freezing in a high applied field (5 T). As the temperature was raised, a step-like decrease in
the sample magnetization was observed, corresponding to the onset of Brownian relaxation …
thawing in the presence of glycerol, a cryo-protectant. For this work we synthesized
magnetite nanoparticles doped with 2.5% cobalt inside the protein ferritin (CMF), dissolved
them in different concentration glycerol solutions and measured their magnetization after
freezing in a high applied field (5 T). As the temperature was raised, a step-like decrease in
the sample magnetization was observed, corresponding to the onset of Brownian relaxation …
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