Validation of RNAi silencing efficiency using gene array data shows 18.5% failure rate across 429 independent experiments
G Munkácsy, Z Sztupinszki, P Herman, B Bán… - … Therapy-Nucleic Acids, 2016 - cell.com
No independent cross-validation of success rate for studies utilizing small interfering RNA
(siRNA) for gene silencing has been completed before. To assess the influence of
experimental parameters like cell line, transfection technique, validation method, and type of
control, we have to validate these in a large set of studies. We utilized gene chip data
published for siRNA experiments to assess success rate and to compare methods used in
these experiments. We searched NCBI GEO for samples with whole transcriptome analysis …
(siRNA) for gene silencing has been completed before. To assess the influence of
experimental parameters like cell line, transfection technique, validation method, and type of
control, we have to validate these in a large set of studies. We utilized gene chip data
published for siRNA experiments to assess success rate and to compare methods used in
these experiments. We searched NCBI GEO for samples with whole transcriptome analysis …
Validation of RNAi Silencing Efficiency Using Gene Array Data shows 18.5% Failure Rate across 429 Independent Experiments
No independent cross-validation of success rate for studies utilizing small interfering RNA
(siRNA) for gene silencing has been completed before. To assess the influence of
experimental parameters like cell line, transfection technique, validation method, and type of
control, we have to validate these in a large set of studies. We utilized gene chip data
published for siRNA experiments to assess success rate and to compare methods used in
these experiments. We searched NCBI GEO for samples with whole transcriptome analysis …
(siRNA) for gene silencing has been completed before. To assess the influence of
experimental parameters like cell line, transfection technique, validation method, and type of
control, we have to validate these in a large set of studies. We utilized gene chip data
published for siRNA experiments to assess success rate and to compare methods used in
these experiments. We searched NCBI GEO for samples with whole transcriptome analysis …
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