We know very little about the vertical distribution of downstream-migrating juvenile Pacific salmon Oncorhynchus spp. in large rivers. It is important for project engineers and fisheries managers to understand the potential interactions of fishes with in-river hydrokinetic devices, which harness a river's energy by spinning a turbine to produce electrical current without damming or impounding water. Currently, several rural Alaskan communities are considering development projects for hydrokinetic devices, including projects in the Tanana River, near Nenana, Alaska. Therefore, the goal of this study was to determine the vertical distribution of juvenile Chinook Salmon Oncorhynchus tshawytscha, Coho Salmon Oncorhynchus kisutch, and Chum Salmon Oncorhynchus keta, in the Tanana River, at a site (bottom depth ∼ 8, channel width ∼ 150 m) where communities may deploy future hydrokinetic turbines. Using a suspended wingless fyke net system during diurnal periods (0800–1800 hours), we found juveniles of all three species at all depths of the water column and no significant differences in catch-per-unit-effort among four depth categories (surface, midwater, deep water, bottom water). The occurrence of juvenile salmon throughout the water column indicates that they may interact with hydrokinetic devices, regardless of their depth. Future research to more comprehensively characterize fish distribution patterns and describe the outcomes of fish–turbine interactions may inform practices aimed at reducing potentially deleterious impacts of hydrokinetic devices on juvenile Pacific salmon.