Very fast CRISPR on demand

Y Liu, R Zou, Y Nihongaki, S He, S Razavi, B Wu… - Biophysical Journal, 2020 - cell.com
CRISPR/Cas systems have enormous potential as tools for genome editing and for studying
DNA damage response through convenient RNA-guided genomic targeting. We developed …

[HTML][HTML] Glen Report 32–24: Very Fast CRISPR (vfCRISPR)“On Demand

G Zon, T Biotechnologies - glenresearch.com
… In modern molecular biology and biotechnology, very few methods have been met with as
much success as CRISPR, as evidenced by this chart of publications indexed to CRISPR in …

[HTML][HTML] “Off-Spotter”: very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs

V Pliatsika, I Rigoutsos - Biology direct, 2015 - Springer
… It appears to the reviewer that the area is evolving fast due to general demand and there
is quite some literature out already. Unfortunately, the authors do not elaborate much about …

In vitro cleavage and electrophoretic mobility shift assays for very fast crispr

RS Zou, Y Liu, T Ha - Bio-protocol, 2021 - bio-protocol.org
… [Abstract] CRISPR-Cas9 has transformed biomedical … through the recently developed very
fast CRISPR (vfCRISPR) … Heating at 95C rapidly inactivates the RNP on demand, allowing …

An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting

J Cao, L Wu, SM Zhang, M Lu… - Nucleic acids …, 2016 - academic.oup.com
… The CRISPR/Cas9 system is a powerful genome editing tool and has been widely used for
… Furthermore, the inducible CRISPR/Cas9 system dramatically decreased off-target effects …

Riding the CRISPR wave

H Ledford - Nature, 2016 - nature.com
… in a single experiment 8 , making it faster and easier to build complicated biological circuits
that … , and in very specific locations in the tissue. “Patterning a blood vessel demands a high …

[PDF][PDF] CRISPR/cas9: an approach to meet global food demand

A Hamza, M Ahmad, A Tariq, AU Din… - Plant Cell …, 2021 - researchgate.net
CRISPR/Cas9 applications for plant genome editing are one of the fast-growing bioscience
technologies. More significantly, CRISPR/Cas9 is evolving into a user-friendly approach to …

[HTML][HTML] CRISPR-based COVID-19 testing: toward next-generation point-of-care diagnostics

U Ganbaatar, C Liu - Frontiers in cellular and infection microbiology, 2021 - frontiersin.org
… As with any emerging technology, CRISPR/Cas-based nucleic … CRISPR/Cas-based detection
methods have the potential to become simpler, more reliable, more affordable, and faster

CRISPR-Cas-mediated diagnostics

L Li, G Shen, M Wu, J Jiang, Q Xia, P Lin - Trends in Biotechnology, 2022 - cell.com
… a faster detection speed with CRISPR diagnostic … of CRISPR proteins that can couple the
detection reaction and readout process also ensure a high level of sensitivity of the CRISPR

[HTML][HTML] CRISPRoff enables spatio-temporal control of CRISPR editing

J Carlson-Stevermer, R Kelso, A Kadina, S Joshi… - Nature …, 2020 - nature.com
… of alleles in the standard sgRNA transfection had already experienced a DSB that was
repaired through NHEJ, confirming our hypothesis that the editing kinetics at this site is very fast. …