Infections are a common post-operative complication associated with high mortality rates after surgical procedures. Coating surgical instruments or implants with antibacterial or antifungal substances may reduce the occurrence rate of infections acquired during surgical operations. The high incidence rate of post-operative infections, caused for example by the implantation of biomedical devices and/or the general hospital environment, has a severe impact on patients’ health. However, no studies have been reported on the prevalence of active species of fungal and bacteria. The present study systematically examines the antibacterial properties (against Staphylococcus, Bacillus, Serratia and Pseudomonas) of nanostructured thin films based on pure TiO2 and TiO2 with Vanadium as an additive, created using a simple and cost effective sol-gel process. The thin films were deposited on glass and Si substrates and their structural and morphological properties were investigated by standard spectroscopic techniques such as X-Ray Diffraction, Scanning Electron Microscopy and Atomic Force Microscopy. The drop test method was used to test for antifungal and antibacterial properties. Our results indicate that increased V concentrations are associated with increased antimicrobial activity in thin films based on TiO2: V.