Visualizing mRNA Dynamics in Live Neurons and Brain Tissues
Localization of mRNA plays a crucial role in a variety of neuronal processes including
synaptogenesis, axonal guidance, and long-term plasticity. Recent advances in
fluorescence imaging and RNA labeling techniques allow us to visualize how individual
mRNA molecules are dynamically regulated inside live neurons and brain tissues. Here, we
describe key methods in imaging mRNA dynamics, including preparation of neuron culture
and brain slices from transgenic mice expressing GFP-labeled mRNA, high-resolution …
synaptogenesis, axonal guidance, and long-term plasticity. Recent advances in
fluorescence imaging and RNA labeling techniques allow us to visualize how individual
mRNA molecules are dynamically regulated inside live neurons and brain tissues. Here, we
describe key methods in imaging mRNA dynamics, including preparation of neuron culture
and brain slices from transgenic mice expressing GFP-labeled mRNA, high-resolution …
Abstract
Localization of mRNA plays a crucial role in a variety of neuronal processes including synaptogenesis, axonal guidance, and long-term plasticity. Recent advances in fluorescence imaging and RNA labeling techniques allow us to visualize how individual mRNA molecules are dynamically regulated inside live neurons and brain tissues. Here, we describe key methods in imaging mRNA dynamics, including preparation of neuron culture and brain slices from transgenic mice expressing GFP-labeled mRNA, high-resolution detection of single molecules, live tissue imaging, and analysis of mRNA transport.
Springer
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