[HTML][HTML] Vitrification affects the expression of matrix metalloproteinases and their tissue inhibitors of mouse ovarian tissue
R Asadzadeh, S Khosravi, S Zavareh… - International Journal …, 2016 - ncbi.nlm.nih.gov
International Journal of Reproductive BioMedicine, 2016•ncbi.nlm.nih.gov
Background: One of the most major obstacles of ovarian tissue vitrification is suboptimal
developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9)
and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the
extracellular matrix in the ovaries. Objective: This study aimed to evaluate the expression of
MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified
mouse ovaries. Materials and Methods: In this experimental study, the gene expression of …
developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9)
and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the
extracellular matrix in the ovaries. Objective: This study aimed to evaluate the expression of
MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified
mouse ovaries. Materials and Methods: In this experimental study, the gene expression of …
Abstract
Background:
One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the extracellular matrix in the ovaries.
Objective:
This study aimed to evaluate the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified mouse ovaries.
Materials and Methods:
In this experimental study, the gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the isolated preantral follicles derived from fresh and vitrified ovaries of 14-16 days old female mice through real time qRT-PCR was evaluated. Developmental parameters, including survival rate, growth, antrum formation and metaphase II oocytes were also analyzed.
Results:
The developmental parameters of fresh preantral follicles were significantly higher than vitrified preantral follicles. The TIMP-1 and MMP-9 expression levels showed no differences between fresh and vitrified preantral follicles (p= 0.22, p= 0.11 respectively). By contrast, TIMP-2 expression significantly decreased (p= 0.00) and MMP-2 expression increased significantly (p= 0.00) in vitrified preantral follicles compared with to fresh ones.
Conclusion:
Changes in expression of MMP-2 and TIMP-2 after ovarian tissues vitrification is partially correlated with decrease in follicle development.
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