Cannabis sativa L. remains under-studied due to years of prohibition and recent research has highlighted the limitations of Cannabis tissue culture methods. Micropropagation and regeneration methods that can reliably be integrated into plant biotechnology pipelines are needed. This thesis explores three aspects of Cannabis tissue culture, which currently lack reliable and replicable methods: the development of high-multiplication rate micropropagation methods, the use of existing leaf-to-plant regeneration method, and the exploration of protoplast-to-plant regeneration systems in Cannabis. The results presented herein, demonstrate that: floral tissues represent a viable alternative to vegetative tissues for the micropropagation of Cannabis; existing methods for regeneration lack the replicability to be used in across a range of representative drug-type genotypes of Cannabis; and that protoplast isolation and cell division from Cannabis callus can be achieved. Taken together, this thesis provides a blueprint for how to design more reliable and replicable Cannabis micropropagation and regeneration methods.