Abstract Analysis of single-cell RNA-Seq data can provide insights into the specific functions
of individual cell types that compose complex tissues. Here, we examined gene expression
in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and
physiologically identified type III cells. Our RNA-Seq libraries met high quality control
standards and accurately captured differential expression of marker genes for type II (eg the
Tas1r genes, Plcb2, Trpm5) and type III (eg Pkd2l1, Ncam, Snap25) taste cells …