Since the 1980s, recombinant human insulin for the treatment of diabetes mellitus has been
produced using either the yeast Saccharomyces cerevisiae or the prokaryote Escherichia
coli. Here, development of the insulin secretory expression system in S. cerevisiae and its
subsequent optimisation is described. Expression of proinsulin in S. cerevisiae does not
result in efficient secretion of proinsulin or insulin. However, expression of a cDNA encoding
a proinsulin-like molecule with deletion of threonine B30 as a fusion protein with the S …

Yeast secretory expression of insulin precursors [7 publications included] … Yeast secretory
expression of insulin precursors [7 publications included] … secrecion; expresion genica;
expression des genes; genie genetique; ingenieria genetica …