Sequencing DNA with nanopores: Troubles and biases

C Delahaye, J Nicolas - PloS one, 2021 - journals.plos.org
… We evaluated the sequencing accuracy of low complexity regions by measuring identity for
… It gives an insight into the actual size of the reads that can be trusted to resolve ambiguities …

Species-specific basecallers improve actual accuracy of nanopore sequencing in plants

S Ferguson, T McLay, RL Andrew, JJ Bruhl… - Plant Methods, 2022 - Springer
… with the aim of improving per-base accuracy. We investigate sequencing accuracies achieved
by ONT basecallers and assess accuracy gains by training single-species and species-…

Sequencing accuracy and systematic errors of nanopore direct RNA sequencing

W Liu-Wei, W van der Toorn, P Bohn, M Hölzer… - BMC genomics, 2024 - Springer
… We present the first comprehensive evaluation of sequencing accuracy and characterisation
… that for sequencing kits SQK-RNA001 and SQK-RNA002, the median read accuracy ranged …

From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy

FJ Rang, WP Kloosterman, J de Ridder - Genome biology, 2018 - Springer
… is its high error rate, which despite recent improvements to the nanopore chemistry … nanopore
sequencing error rate. Furthermore, we outline strategies for translation of raw sequencing

Nanopore sequencing in a clinical routine laboratory: challenges and opportunities.

J Schmidt, F Blessing, L Fimpler… - Clinical …, 2020 - search.ebscohost.com
… However, the actual number of working nanopores on the sensor array is a … accuracy and
performance in general. If this trend continues, one can assume that nanopore sequencing

Is Oxford Nanopore sequencing ready for analyzing complex microbiomes?

LJ Kerkhof - FEMS microbiology ecology, 2021 - academic.oup.com
… for the various SSU genes compared with the actual target abundance within the sample.
They also … researchers to improve nanopore sequence accuracy and genome reconstruction. …

Error analysis of idealized nanopore sequencing

CR O'Donnell, H Wang, WB Dunbar - Electrophoresis, 2013 - Wiley Online Library
… imposed by the instrument and enzyme rate in actual experiments. Example current traces
show the … To identify an accurate metric of base quality for a nanopore sequencing method, …

Oxford Nanopore sequencing: new opportunities for plant genomics?

K Dumschott, MHW Schmidt, HS Chawla… - Journal of …, 2020 - academic.oup.com
sequencing technologies (third-generation sequencing) can produce reads that are several
kilobases in length. This greatly improves the accuracy … ’ or the actual accuracy of ~99.999% …

Clinical performance of nanopore targeted sequencing for diagnosing infectious diseases

Y Fu, Q Chen, M Xiong, J Zhao, S Shen… - Microbiology …, 2022 - Am Soc Microbiol
… together and sequenced from end to end in nanopore sequencing with … actual accuracy
rate of NTS assay is higher than validation rate provided by PCR followed by Sanger sequencing

Sequencing of human genomes with nanopore technology

R Bowden, RW Davies, A Heger… - Nature …, 2019 - nature.com
… data and observed results in the actual data again indicate that … the clinical utility of nanopore
sequencing technology for an … evaluation of the accuracy of ONT sequencing for variant …