[HTML][HTML] Advances in the analysis of single extracellular vesicles: A critical review

SH Hilton, IM White - Sensors and actuators reports, 2021 - Elsevier
Extracellular vesicles (EVs) recovered from a liquid biopsy … New approaches have emerged
to analyze EVs at the single … describe and analyze methods for single EV analysis that have …

Sequencing-based protein analysis of single extracellular vesicles

J Ko, Y Wang, K Sheng, DA Weitz, R Weissleder - ACS nano, 2021 - ACS Publications
… The same approach was used for future analysis. seiSEQ of Macrophage-Derived
Vesicles To show the potential for multiplexed single EV analysis, we performed next-generation …

Multiplexed profiling of single extracellular vesicles

K Lee, K Fraser, B Ghaddar, K Yang, E Kim, L Balaj… - ACS …, 2018 - ACS Publications
… In this paper we describe a single EV analysis (SEA) technique that is capable of robust,
multiplexed protein biomarker measurement in individual vesicles. We first immobilize EV …

Analysis of single extracellular vesicles for biomedical applications with especial emphasis on cancer investigations

T Wang, Y Xing, Z Cheng, F Yu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
… of single EVs and their sensitive detection are urgently demanded. In this article, we survey
the latest progress in single EVs analysis … and labelling approaches of single EVs detection. …

Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms

T Arab, ER Mallick, Y Huang, L Dong… - … extracellular vesicles, 2021 - Wiley Online Library
We compared four orthogonal technologies for sizing, counting, and phenotyping of
extracellular vesicles (EVs) and synthetic particles. The platforms were: single‐particle …

Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material

A Görgens, M Bremer, R Ferrer-Tur… - … extracellular vesicles, 2019 - Taylor & Francis
… for a brightness range more suitable for cellular analysis. Thus, there is a general consensus
in … for the detection of single EVs. To optimise parameters for single EV analysis, we used …

Extracellular vesicles: techniques and biomedical applications related to single vesicle analysis

J Zhang, J Wu, G Wang, L He, Z Zheng, M Wu… - ACS …, 2023 - ACS Publications
… In recent years, the evolution of single EV (SEV) analysis has enabled an in-depth … the
individual vesicle level. This review examines the sample acquisition tactics prior to SEV analysis, …

EV-Ident: identifying tumor-specific extracellular vesicles by size fractionation and single-vesicle analysis

D Kim, HK Woo, C Lee, Y Min, S Kumar… - Analytical …, 2020 - ACS Publications
… size fractions of nanoparticles for analysis of a size-specific single extracellular vesicle (EV).
(… The expression levels of biomarkers in individual EVs are analyzed by single-EV analysis. …

Multiplexed profiling of single-cell extracellular vesicles secretion

Y Ji, D Qi, L Li, H Su, X Li, Y Luo… - Proceedings of the …, 2019 - National Acad Sciences
… To further demonstrate potential applications of a single-cell EV analysis platform to clinical
… We applied the single-cell analysis workflow to primary tumor cells and generated excellent …

Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles

G Bordanaba-Florit, F Royo, SG Kruglik… - Nature …, 2021 - nature.com
… Recent developments in single-vesicle analysis have made … current methods for the analysis
of single vesicles and review the … potential of innovative single-vesicle imaging and analysis