… Antibodies are composed of two types of protein chain, known as the heavy and light chains
(Fig. 1). Each of these is encoded by multiple gene segments that are spliced together using …

… In a separate experiment designed to verify native VH-VL pairing accuracy, we transfected
… True VH-VL pairs cannot be known for an entire human antibody repertoire, but we can …

… have managed to discard the need for a light chain in their repertoire of VHH antibodies. …
can address a particular epitope with the likelihood of forming a viable antibody-antigen pairing …

… SLC-pairing μHCs increases the primary antibody repertoire, … antibody specificity. Since
proliferation of pre-B cells is only accomplished by the presence of a complete pre-BCR, pairing …

… H and V L ) allow isolation of antibodies against virtually any antigen.3, 4… pairing of V H and
V L domains, which excludes information about the cognate pairing of heavy and light chains…

… repertoire is dominated by a limited number of pairings of segments and folds. Among these
pairings we identified two identical mutated heavy chains … different mutated light chains (one …

… their light chain pairing, but NGS could not elucidate the natural heavy and light chain pairings
(… natural functional heavy and light chain pairings, but mismatched pairings led to greater …

… Heavy chain pairing with diverse light chains is known to be quite promiscuous, based on
extensive data from phage display library experiments. Therefore, it is challenging to select …

… antibody repertoire is fundamental to our understanding of B-cell immunity. We sequenced
the paired heavy- and light-chain … Convergence in light-chain gene pairing was markedly …

… pairing algorithm only predicts modellable Fv pairings based on PDB structures, we compared
our IGHV/IG[K/L]V pairing … is limited to the antibody chains that are currently predicted to …