… determining HCMV genome sequences directly from infected cell cultures without purifying
… virus in cell culture. The six HCMV strains targeted included four grown in cell culture (3157 …

… To globally assess viral gene function for replication in cells, we determined the genomic …
The growth of these mutants in different cultured cells was examined to systematically …

… We set out to paint a full picture of what happens dynamically to entire HCMV genomes
when adapted from clinical samples to cell culture. Our aim was to identify patterns of mutation …

… in culture. We have determined the sequence (241 087 bp) of chimpanzee cytomegalovirus
(… in the urine of a congenitally infected child (ie not passaged in cell culture) were amplified. …

… In cell culture, persistent infection by a genital isolate of CMV has resulted in … for the CMV
genome (14) and 3.92 x 1012 for the host cell genome (15). Known amounts of unlabeled CMV …

… Fibroblasts, epithelial and endothelial cells were infected in parallel with three low-… cell
cultures were then passaged weekly 50–63 times, before sequencing the complete genome of …

… cell culture, so CMV is … CMV genome at other replicative stages remains poorly defined. For
example, it is not known whether, during latency, the CMV genome is integrated into host cell …

… in cell culture. Comparative analyses, which employed the published genome sequence of
a … passaged in cell culture, we assess the gene content and variability of wild-type HCMV. …

… from clinical isolates through both Sanger sequencing of overlapping PCR products and
NGS analysis of infected cell cultures and unamplified clinical material with the …

… dominant polypeptides in particles purified from cell culture supernatant. Although its gene is
… A cluster of dispensable genes within the human cytomegalovirus genome short component…