… site of vulnerability on SARS-CoV-2 spike. These antibodies prevent viral receptor engagement
by locking the receptor-binding domain of spike in the down conformation, revealing a …

… -CoV-1 S and its function remains poorly understood. Here we engineered a SARS-CoV-2
S protein construct “SR/x3” to arrest SARS-CoV-2 spikes in the locked conformation by a …

… d The mAbs neutralize all current SARS-CoV-2 VOCs by locking RBD in down
conformation d 12-19 escape mutations are rarely found in circulating SARSCoV-2 viruses …

… We propose that the locked-1 and locked-2 conformations of S are present in the acidic
high-lipid cellular compartments during virus assembly and egress. In this model, release of the …

… SARS coronavirus (SARS-CoV) was identified as the etiological agent of the pandemic
transmissible disease severe acute respiratory syndrome (SARS). During the outbreak in 2003, …

… and exhibited two conformations: the locked conformation and the active conformation, with
overall resolutions of 2.7 Å and 3.3 Å, respectively. The locked conformation is similar to the …

… the binding site and by locking the RBDs into an inactive conformation. Although a multitude
of other monoclonal and single-domain antibodies against SARS-CoV-2 Spike have been …

… But our spike protein conformation is very different from the previously … SARS-CoV-2 spike
protein and ACE2 monomer indicate that monomer ACE2 binding can induce conformational …

… We were unable to find any such states, suggesting that the disulfide bond between the RBD
and the S2 subunit effectively locked the SARS-CoV-2 spike in its down conformation. The …

… interest in locking the S trimer into the prefusion conformation. … the prefusion conformation
of the SARS-CoV and MERS-CoV … did not appear to inhibit RBD motion in SARS-CoV-2 (24), …